replica plating

replica plating
Technique for testing the genetic characteristics of bacterial colonies. A dilute suspension of bacteria is first spread, in a petri dish, on agar containing a medium expected to support the growth of all bacteria, the master plate. Each bacterial cell in the suspension is expected to give rise to a colony. A sterile velvet pad, the same size as the petri dish, is then pressed onto it, picking up a sample of each colony. The bacteria can then be "stamped" onto new sterile petri dishes, plates, in the identical arrangement. The media in the new plates can be made up to lack specific nutritional requirements or to contain antibiotics. Thus colonies can be identified that cannot grow without specific nutrients or that are antibiotic-resistant, and cells with mutations in particular genes can be isolated.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

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  • replica plating — A technique for isolating mutants from a population by plating cells from each colony growing on a nonselective agar medium onto plates with selective media or environmental conditions, such as the lack of a nutrient or the presence of an… …   Dictionary of microbiology

  • replica plating — Duplicating a population of bacterial colonies growing on agar medium in one Petri plate to agar medium in another Petri plate …   Glossary of Biotechnology

  • replica plating [method] — replica plating [method]. См. метод отпечатков. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • plating — 1. Sowing of bacteria on a solid medium in a Petri dish or similar container; the making of a plate culture. 2. Application of a metal bar to keep the ends of a fractured bone in apposition. 3. Electrolytic deposition of a …   Medical dictionary

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  • Реплика метод отпечатков — Реплика, метод отпечатков * рэпліка, метад адбіткаў * replica or replica plating or r. method 1. Метод оперативного выявления биохимических мутаций у микроорганизмов. Получают отпечаток бактериальной колонии (в оригинале метода E. coli),… …   Генетика. Энциклопедический словарь

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